Takeaway
Chip-based digital polymerase chain reaction (PCR) of cell-free tumor DNA (ctDNA) extracted from the cerebrospinal fluid (CSF) can be used to molecularly diagnose gliomas with high sensitivity and specificity.
Why this matters
The World Health Organization (WHO) classification of gliomas requires invasive biopsies from deep brain tissues; however, this study supports that new technologies like chip-based digital PCR may allow for non-invasive molecular diagnosis of diffuse gliomas.